Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Characterization of 16S rRNA mutations that decrease the fidelity of translation initiation.

Daoming Qin1, Nimo M Abdi, Kurt Fredrick

  • 1Ohio State Biochemistry Program, The Ohio State University, Columbus, Ohio 43210, USA.

RNA (New York, N.Y.)
|October 19, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Role of the anti-Shine-Dalgarno sequence of 16S rRNA in Flavobacterium johnsoniae.

Nucleic acids research·2026
Same author

Structure of a specialized 70S initiation complex.

Nucleic acids research·2026
Same author

Ribosome deficiency induces <i>Salmonella</i> filamentation within host cells.

mBio·2025
Same author

Protein bL38 facilitates incorporation of uL6 during assembly of the 50S subunit in Flavobacterium johnsoniae.

Nucleic acids research·2025
Same author

Role of the sarcin-ricin loop of 23S rRNA in biogenesis of the 50S ribosomal subunit.

RNA (New York, N.Y.)·2025
Same author

Structural analysis of noncanonical translation initiation complexes.

The Journal of biological chemistry·2024
Same journal

Optimized tRNA structure-seq reveals robust tRNA secondary structures in <i>S. cerevisiae</i> under mild stress conditions.

RNA (New York, N.Y.)·2026
Same journal

SERIPH: A Two-Step Extraction Protocol for Selective Enrichment of Semi-Extractable RNAs.

RNA (New York, N.Y.)·2026
Same journal

Reduced Sensitivity to RNA Structural Differences Distinguishes Eukaryotic Pus4 from Bacterial TruB.

RNA (New York, N.Y.)·2026
Same journal

Puf3 contributes to changes in mRNA solubility, translation elongation dynamics at rare arginine codons and loss of protein homeostasis in cells lacking Not4.

RNA (New York, N.Y.)·2026
Same journal

RBM38 Regulates HORMAD1 Splicing to Enhances MEK Inhibitor Sensitivity in Breast Cancer.

RNA (New York, N.Y.)·2026
Same journal

EF-P Inhibits Ribosomal α-Hydroxy Acid Incorporation: Strategic tRNA Body Selection for Co-incorporating α-Hydroxy Acids and Nonproteinogenic Amino Acids into Depsipeptides.

RNA (New York, N.Y.)·2026
See all related articles

Bacterial translation initiation fidelity is reduced by two 16S rRNA mutations. G1338A increases initiator tRNA binding, while A790G affects initiation factor 3 binding, both leading to more errors.

Area of Science:

  • Molecular Biology
  • Bacterial Genetics
  • Ribosome Function

Background:

  • Bacterial translation initiation is a complex process regulated by initiation factors (IF1, IF2, IF3) and the 30S ribosomal subunit.
  • Accurate selection of the initiator tRNA and start codon is crucial for correct protein synthesis.
  • 16S ribosomal RNA (rRNA) plays a key role in ribosome structure and function, including translation initiation.

Purpose of the Study:

  • To investigate the in vivo effects of specific 16S rRNA mutations (G1338A and A790G) on bacterial translation initiation fidelity.
  • To elucidate the distinct mechanisms by which these mutations impair accurate start codon selection.

Main Methods:

  • In vivo genetic analysis of bacterial strains harboring specific 16S rRNA mutations.

Related Experiment Videos

  • Assessing translation initiation fidelity by measuring spurious initiation events.

    • Main Results:

    • The G1338A mutation in 16S rRNA enhances the binding affinity of the initiator tRNA (fMet-tRNA(fMet)) to the 30S subunit.
    • The A790G mutation decreases the affinity of initiation factor 3 (IF3) for the 30S subunit.
    • Both mutations lead to decreased translation initiation fidelity, resulting in increased spurious initiation.

    Conclusions:

    • The G1338A mutation may compensate for codon-anticodon mismatches, leading to increased initiation errors.
    • The A790G mutation's effect on IF3 or 50S subunit docking contributes to reduced initiation fidelity.
    • These findings highlight the critical roles of specific 16S rRNA residues in ensuring accurate translation initiation in bacteria.