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Updated: Jul 10, 2026

Polymerase Chain Reaction and Dot-Blot Hybridization for Leptospira Detection in Water Samples
Published on: June 14, 2024
Molecular detection of Leptospira interrogans in human tissues and environmental samples in a lethal case of
D Luchini1, F Meacci, M R Oggioni
1Department of Forensic Sciences, University of Siena, Policlinico Le Scotte, Viale Bracci, 53100 Siena, Italy. luchini2@unisi.it
Abstract:
A forensic case of suspected Leptospirosis with fatal course was resolved by the molecular detection of Leptospira interrogans in postmortem human tissues and in environmental samples. Polymerase chain reaction analysis and DNA sequencing confirmed the clinical diagnosis of Weil syndrome, and the death was considered to be an occupational accident with all the legal implications.
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