The rat liver epithelial (RLE) cell protein database.

Area of Science:

• Proteomics
• Cell Biology
• Bioinformatics

Background:

• Rat liver epithelial (RLE) cells are a model for studying cellular processes.
• Understanding protein expression is crucial for cell biology research.
• Existing protein databases may lack detailed information on specific cell types or conditions.

Purpose of the Study:

• To establish comprehensive computer databases of rat liver epithelial (RLE) cellular polypeptides.
• To analyze protein expression patterns in normal and transformed RLE cells.
• To create a resource for studying RLE cell growth, differentiation, and transformation.

Main Methods:

• High-resolution two-dimensional gel electrophoresis (2D-PAGE) for polypeptide separation.
• Computer-assisted analysis using the Elsie 5 gel analysis system.
• Subcellular fractionation to create detailed protein maps of various cellular components.

Main Results:

• Databases were constructed for [35S]methionine-labeled, [32P]orthophosphate-labeled, and silver-stained RLE polypeptides.
• Analysis included whole cell lysates, cytosolic, nuclear, membrane-associated, and mitochondrial fractions.
• Databases contain qualitative and quantitative data, including protein identification, molecular weight, pI, and transformation-sensitive markers.

Conclusions:

• The established RLE databases provide a valuable resource for proteomic research.
• These databases facilitate the study of protein regulation in RLE cells.
• The findings support future investigations into RLE cell growth, differentiation, and transformation.