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Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI
Published on: July 12, 2012
Fractionated manganese-enhanced MRI.
Nicholas A Bock1, Fernando F Paiva, Afonso C Silva
1Cerebral Microcirculation Unit/Laboratory of Functional and Molecular Imaging/National Institute of Neurological Disorders and Stroke/National Institutes of Health, Bethesda, MD 20892-1065, USA. bockn@mail.nih.gov
NMR in Biomedicine
|October 19, 2007
Summary
Fractionated manganese-enhanced MRI (MEMRI) uses smaller, repeated doses of manganese chloride to enhance brain imaging signals in rats. This method balances manganese
Area of Science:
- Neuroimaging
- Biomedical Engineering
- Pharmacology
Background:
- Manganese-enhanced MRI (MEMRI) utilizes manganese chloride as a neuronal contrast agent.
- High doses of manganese can cause toxicity, limiting its use.
- Fractionated dosing strategies are explored to mitigate manganese toxicity.
Purpose of the Study:
- To investigate fractionated dosing of manganese chloride for MEMRI in animal models.
- To balance the contrast-enhancing properties of manganese with its potential toxic effects.
- To optimize manganese delivery for improved neuronal imaging.
Main Methods:
- Rats received fractionated doses of manganese chloride (MnCl2·4H2O) every 48 hours.
- T1-weighted imaging was used to monitor signal enhancement in the brain.
- T1 mapping assessed manganese accumulation and its relationship to fraction dose and concentration.
Main Results:
- Signal enhancement increased with consecutive fractionated doses and eventually saturated.
- Lower T1 values (indicating higher manganese concentration) were observed with increasing fraction doses for a fixed cumulative dose.
- Adverse systemic health effects and injection site complications increased with higher fraction doses.
Conclusions:
- Fractionated MEMRI allows for controlled manganese accumulation, retaining its contrast properties.
- This approach offers a method to mitigate manganese toxicity in animal studies.
- Fractionated dosing provides a viable strategy for balancing efficacy and safety in manganese-enhanced neuroimaging.
