[Quantitative determination of glucose by internal standard laser Raman spectra]

In the present paper we report quantitative analysis of glucose using internal standard laser Raman spectra. A good linear correlation was observed between the intensities of the -COO band at 1 125 cm(-1) using excition wavelength of 632.81 nm (r = 0.998 8) and the glucose concentration over the range 0-1.8 mol x L(-1). Band intensities were normalized against an internal standard (water band at 1 643 cm(-1)), and the limit of detection (L. O. D.) of glucose is 0.022 7 mol x L(-1). The interference ions would not influence the quantitative analysis. When this method was used to determine 5% glucose NaCl, 5% glucose, and 10% glucose injections, the result showed that the recoveries are 71.88%-126.31%, 81.02%-124.89% and 74.87%-121.32%, and the RSDs are 5.44%, 4.34% and 0.94%, respectively. The non destructive, non intrusive nature of the method makes internal standard laser Raman spectra a convenient, accurate, and green quantitative analysis method.