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Related Concept Videos

Cholera01:25

Cholera

Cholera is an acute gastrointestinal disease caused by the Gram-negative bacterium Vibrio cholerae. It is transmitted primarily via the fecal-oral route through the ingestion of contaminated water or food.Vibrio cholerae is a motile, Gram-negative bacterium of the family Vibrionaceae, primarily associated with waterborne outbreaks in areas with inadequate sanitation. Although over 200 serogroups of V. cholerae exist, only O1 and O139 are responsible for epidemic cholera. The O1 serogroup,...
Antibiotic Selection00:57

Antibiotic Selection

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Related Experiment Video

Updated: Jul 10, 2026

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
07:58

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates

Published on: May 30, 2017

Selective chromosome amplification in Vibrio cholerae.

Preeti Srivastava1, Dhruba K Chattoraj

  • 1Laboratory of Biochemistry and Molecular Biology, Center for Cancer Research, NCI, NIH, Bethesda, MD 20892-4260, USA.

Molecular Microbiology
|October 20, 2007
PubMed
Summary

Bacterial growth rate affects chromosome replication differently. Vibrio cholerae

Area of Science:

  • Microbiology
  • Bacterial Genetics
  • Molecular Biology

Background:

  • Most bacteria possess a single chromosome, unlike eukaryotes which commonly have multiple.
  • The mechanisms governing the maintenance of multiple chromosomes in bacteria are not well understood.

Purpose of the Study:

  • To investigate the replication behavior of the two Vibrio cholerae chromosomes in response to varying growth rates.
  • To understand how chrI and chrII copy numbers are regulated under different nutritional conditions.

Main Methods:

  • Comparative analysis of chromosome copy numbers at different bacterial growth rates.
  • Genetic manipulation involving excess initiator supply and regulator deletion for chrII.
  • Testing plasmid replication behavior in V. cholerae.

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TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
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Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species
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Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species

Published on: November 8, 2016

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Last Updated: Jul 10, 2026

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
07:58

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Published on: May 30, 2017

TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
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Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species
09:34

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species

Published on: November 8, 2016

Main Results:

  • At slow growth rates, both Vibrio cholerae chromosomes (chrI and chrII) are present at 1-2 copies per cell.
  • Nutritional shift-up leads to amplification of chrI but not chrII.
  • chrII amplification occurs with excess initiator or regulator deletion, showing growth rate-insensitive replication similar to some plasmids, but is cell-cycle regulated.
  • Increased chrII copy number negatively impacts bacterial growth rate.

Conclusions:

  • Vibrio cholerae chrI replication is growth rate-dependent, while chrII exhibits growth rate-insensitive replication.
  • chrII's replication strategy resembles that of certain plasmids but is distinct in its cell-cycle regulation.
  • The data suggest chrII may function as a reservoir for essential but potentially harmful genes, with its copy number regulation impacting bacterial fitness.