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Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR
Published on: February 1, 2014
Occurrence of Escherichia coli O157 on hides of slaughtered cattle
I Nastasijevic1, R Mitrovic, S Buncic
1Institute of Meat Hygiene and Technology, Belgrade, Serbia.
Aims:
To obtain the first information on the occurrence of Escherichia coli O157 on hides of slaughtered cattle in Serbia.
Methods And Results:
A total of 355 swabs were taken on the slaughterline from five areas of hide of each of the 71 cattle in a single commercial abattoir in Serbia. Using an ISO method incorporating enrichment and immunomagnetic separation steps, E. coli O157 was isolated from the hides of 20 animals (28 x 2%). With respect to different areas of the hides, the occurrence of the pathogen was, in decreasing order: hooves (11 x 3%), brisket (8 x 4%), rump (7 x 0%), neck (4 x 2) and flank (2 x 8%). In addition, factors that had more or less effects on the occurrence included visible dirtiness of the hide, cattle's age category, geographical origin of the animals and season.
Conclusions:
This study revealed the presence of E. coli O157 in the beef chain in Serbia and confirmed hide as an important potential source of related contamination of beef carcasses. Therefore, incorporation of preskinning hide decontamination treatments into HACCP-based slaughterline hygiene control measures could be very useful.
Significance And Impact Of The Study:
The results will enable further optimization of necessary measures along the beef chain to reduce the E. coli O157 risks in Serbia.
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