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Updated: Jul 10, 2026

Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry
Published on: April 4, 2018
Analysis of underivatized polyamines by reversed phase liquid chromatography with electrospray tandem mass
Merja R Häkkinen1, Tuomo A Keinänen, Jouko Vepsäläinen
1Department of Biosciences, Laboratory of Chemistry, University of Kuopio, PO Box 1627, FI-70211 Kuopio, Finland. merja.hakkinen@uku.fi
Abstract:
A reversed phase liquid chromatography-electrospray ionization-tandem mass spectrometric method (RP-LC-ESI-MS/MS) was developed to separate and detect polyamines with minimal sample pre-treatment and without any derivatization. Prior to MS/MS analysis, a complete chromatographic separation of polyamines was achieved by a linear gradient elution using heptafluorobutyric acid as a volatile ion-pair modifier, and signal suppression was prevented by post-column addition of 75% propionic acid in isopropanol to the column flow. The developed method was successfully applied to the identification of metabolites formed from N(1),N(12)-diethylspermine in the reaction catalyzed by recombinant human polyamine oxidase and to the detection of eight different polyamines in a standard mixture.
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