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Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
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Quantification of brain creatine concentration using PRESS sequence and LCModel: comparison with HPLC method.

Y Lin1, Y P Zhang, Z W Xiao

  • 1Dept. of Med. Imaging, Shantou Univ. Med. Sch. Shantou , Guangdong, 515041, China.

Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference
|October 20, 2007
PubMed
Summary

This study demonstrates that long echo time PRESS magnetic resonance spectroscopy (MRS) with an external standard accurately quantifies brain total creatine (Cr) concentration. LCModel software enhances the convenience of this method for reliable in vivo measurements.

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Area of Science:

  • Neuroimaging
  • Biochemistry
  • Medical Physics

Background:

  • Accurate quantification of brain metabolites like total creatine (Cr) is crucial for understanding neurological conditions.
  • In vivo proton magnetic resonance spectroscopy (1H-MRS) is a non-invasive technique for measuring brain metabolites.
  • External standards and advanced modeling software can improve the accuracy of MRS quantification.

Purpose of the Study:

  • To evaluate the accuracy of a long echo time (TE) PRESS sequence combined with an external standard and LCModel for quantifying brain total creatine (Cr) concentration in vivo.
  • To compare the in vivo MRS results with in vitro high-performance liquid chromatography (HPLC) measurements.

Main Methods:

  • Ten swine and an external standard were studied using a 1.5 T GE Signa scanner.
  • Single-voxel 1H-MRS data were acquired from the swine brain and external standard using a PRESS sequence (TE=135 ms, TR=1500 ms, 128 averages).
  • Quantification of Cr was performed using the Linear Combination of Model Spectra (LCModel) software. In vitro Cr concentration was determined by HPLC post-mortem.

Main Results:

  • The mean brain Cr concentration measured by MRS was 9.37 ± 0.137 mmol/kg.
  • The mean brain Cr concentration measured by HPLC was 8.905 ± 0.126 mmol/kg.
  • No statistically significant difference was found between the MRS and HPLC measurements (P=0.491).

Conclusions:

  • A long TE PRESS sequence with an external standard provides accurate in vivo quantification of brain total creatine concentration.
  • The LCModel software offers a convenient approach for MRS-based metabolite quantification.
  • This validated method holds promise for non-invasive assessment of brain creatine levels in research and clinical settings.