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Published on: January 19, 2015
Electroporation-induced cell lysis in SWLA-2 hybridomas
Barbara A Oakley1, Brooks A Gross, Sara G Martens
1Sch. of Eng. & Comput. Sci., Oakland Univ., Rochester, MI 48309, USA. oakley@oakland.edu
Summary
High voltage electric pulses can induce significant cell lysis in hybridoma cells. Applying three 0.6 ms pulses at 300 V resulted in nearly 90% cell mortality, demonstrating effective electropermeabilization.
Area of Science:
- Biotechnology
- Cell Biology
- Electrochemistry
Background:
- Hybridoma cell lines are crucial for monoclonal antibody production.
- Electroporation is a method used to introduce substances into cells.
- Optimizing electroporation parameters is key for efficient cell manipulation.
Purpose of the Study:
- To investigate the effect of varying electric field pulse parameters on SWLA-2 murine hybridoma cell lysis.
- To determine the optimal conditions for achieving high cell mortality using square wave electric pulses.
Main Methods:
- SWLA-2 murine hybridomas were subjected to square wave electric field pulses.
- Parameters varied included voltage (100-300 V), pulse duration (0.2, 0.6 ms), and number of pulses (1-3).
- Cell lysis was assessed 48 hours post-treatment by examining cell mortality.
Main Results:
- Nearly 90% cell mortality was achieved.
- Optimal conditions involved applying 3 pulses of 0.6 ms duration at 300 V.
- Cell lysis percentage was dependent on voltage, pulse duration, and number of pulses.
Conclusions:
- Square wave electric field pulses are effective in inducing significant cell lysis in murine hybridomas.
- Specific parameters (300 V, 0.6 ms, 3 pulses) yield high cell mortality.
- This study provides valuable data for optimizing electroporation protocols in hybridoma cell culture.
