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[Effect of migillin on protein synthesis in intact and virally infected TKCE and FL cells]
Abstract:
The effect of migillin was studied with respect to protein synthesis in the cells of TKCE (transplantable line of cells of the kidneys of cow embryos) and FL--intact and infected with viruses of aphtous fever A22, strain 550 and poliomyelitis of type III, strain Saukett respectively. Simultaneously the effect of migillin on sensitivity of the cells to the above viruses was tested. The synthesis of protein was determined by incorporation of C14-glycine into the acid insoluble fraction of the cells. It was found that protein synthesis significantly increased under the effect of migillin in both the intact cells and the cells infected with the viruses. The viruses of aphtous fever and poliomyelitis inhibited the synthesis of proteins. The antibiotic increased the activity of dehydrogenases and respiration of HEp-2 cells. Migillin markedly suppressed reproduction of the poliomyelitis virus, the effect on the virus of aphtous fever was lower in the cell culture. In guinea pigs the antiviral effect of the antibiotic resulted in prolongation of the incubation period and retardation of the aphtous fever process generalization. The results of the experiments showed that migillin stimulated the activity of dehydrogenases, respiration, protein synthesis in the cell cultures and possessed antiviral activity in vitro and in vivo.
Insights
Migillin enhances protein synthesis and cell activity, while also exhibiting antiviral properties against poliomyelitis and aphtous fever viruses in cell cultures and animal models.
Area of Science:
- Biochemistry
- Virology
- Cell Biology
Background:
- Protein synthesis is crucial for cellular function and is often targeted by viral infections.
- Understanding how external agents affect cellular processes and viral replication is key to developing therapeutics.
Purpose of the Study:
- To investigate the impact of migillin on protein synthesis in various cell lines.
- To evaluate migillin's effect on cellular sensitivity to viral infections.
- To assess the antiviral efficacy of migillin in vitro and in vivo.
Main Methods:
- Protein synthesis was measured by C14-glycine incorporation into acid-insoluble cell fractions.
- Cellular dehydrogenases activity and respiration were monitored.
- Viral reproduction was assessed in cell cultures and in vivo models (guinea pigs).
Main Results:
- Migillin significantly increased protein synthesis in both intact and virus-infected TKCE and FL cells.
- Viruses of aphtous fever and poliomyelitis inhibited protein synthesis, an effect counteracted by migillin.
- Migillin suppressed poliomyelitis virus reproduction more effectively than aphtous fever virus in cell culture.
- In vivo, migillin prolonged incubation periods and slowed disease progression in guinea pigs with aphtous fever.
Conclusions:
- Migillin stimulates cellular dehydrogenases activity, respiration, and protein synthesis.
- The antibiotic demonstrates significant antiviral activity against poliomyelitis and aphtous fever viruses.
- Migillin shows potential as a therapeutic agent for viral infections.