Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Bioreactor Design and Operational System

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...

Bioreactor Controls-II

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...

Upstream Processing

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Table Tennis as a Tool for Physical Education and Health Promotion in Primary Schools: A Systematic Review.

Sports (Basel, Switzerland)·2025

Same author

Fever management with or without a temperature control device after out-of-hospital cardiac arrest and resuscitation (TEMP-CARE): A study protocol for a randomized clinical trial.

Acta anaesthesiologica Scandinavica·2025

Same author

Cancer diagnosis in Catalonia (Spain) after two years of COVID-19 pandemic: an incomplete recovery.

ESMO open·2022

Same author

A new approach for initial callus growth during fracture healing in long bones.

Computer methods and programs in biomedicine·2021

Same author

FAIR data sharing: The roles of common data elements and harmonization.

Journal of biomedical informatics·2020

Same author

Biomechanical analysis of the stability of anterograde reamed intramedullary nails in femoral spiral fractures.

Injury·2020

Search research articles

Related Experiment Video

Updated: Jul 10, 2026

Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells

Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells

Published on: September 28, 2018

Multiple automated minibioreactor system for multifunctional screening in biotechnology.

A Fontova¹, A Soley, J Gálvez

¹Electronic Engineering Dept., Technical University of Catalonia, Barcelona, Spain.

Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference

|October 20, 2007

Summary

This study introduces a novel multiwell screening system that mimics industrial bioreactor conditions for cell culture testing. This innovation bridges the gap between high throughput screening and pre-industrial scale-up, improving biotechnological molecule testing.

More Related Videos

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation

Published on: May 18, 2020

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

Related Experiment Videos

Last Updated: Jul 10, 2026

Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells

Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells

Published on: September 28, 2018

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation

Published on: May 18, 2020

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

Area of Science:

Biotechnology
Cell Culture Technology
Bioprocess Engineering

Background:

High throughput screening (HTS) is crucial for testing molecules but differs significantly from pre-industrial bioreactor conditions.
Current methods involve manual processes in larger bioreactors, creating a gap between screening and scale-up.
Reproducing industrial bioreactor conditions at the screening level is essential for efficient bioprocess development.

Purpose of the Study:

To develop a novel screening system that replicates industrial bioreactor conditions.
To enable accurate testing of molecules using cell cultures under scalable conditions.
To bridge the gap between HTS and pre-industrial scale-up in biotechnology.

Main Methods:

A multiwell test plate system designed as disposable multiple minibioreactors.
Replication of key industrial bioreactor parameters: aeration, stirring, temperature, O2, and pH.
Capability to perform visible range optical absorbance measurements.
Adaptable for both suspended and adherent animal cell types in 1 to 10 ml volumes.

Main Results:

The system successfully reproduces industrial bioreactor culture conditions in a multiwell format.
It allows for the testing of both suspended and adherent animal cell types.
The minibioreactors enable precise control over critical growth parameters.
Limitations were observed for achieving high bacterial or yeast biomass due to air supply constraints.

Conclusions:

The developed screening system effectively mimics industrial bioreactor conditions for cell culture.
It offers a valuable tool for optimizing bioprocess development and molecule testing.
The system enhances the transition from high throughput screening to pre-industrial scale-up for animal cell cultures.