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Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling
Published on: December 12, 2014
Cutting edge: Atypical PKCs regulate T lymphocyte polarity and scanning behavior
Eliana Real1, Sophie Faure, Emmanuel Donnadieu
1Institut Cochin, Université Paris Descartes, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8104, Paris, France.
Journal of Immunology (Baltimore, Md. : 1950)
|October 20, 2007
Summary
Atypical protein kinases C (aPKCs) are crucial for T cell polarization and uropod formation, essential for immune cell motility and interaction with other cells. These findings reveal a key signaling pathway regulating lymphocyte movement.
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- Leukocyte locomotion is a polarized cellular process.
- Mechanisms generating T cell asymmetry downstream of chemokine receptors are not well understood.
Purpose of the Study:
- To investigate the role of atypical protein kinases C (aPKCs) in T cell polarization and motility.
- To elucidate the signaling pathways involved in establishing T cell asymmetry.
Main Methods:
- Investigated the function of aPKCs (PKCiota and PKCzeta) in T cell symmetry breaking and polarity.
- Examined the interaction between Par6 and aPKCs.
- Assessed the impact of impaired aPKC signaling on T cell motility and dendritic cell scanning.
Main Results:
- aPKCs are required for early symmetry breaking in T cells.
- aPKCs regulate uropod formation and depend on Par6 interaction.
- Reduced aPKC signaling impairs T cell motility and scanning of dendritic cells.
Conclusions:
- aPKCs play a critical role in establishing T cell polarity and regulating lymphocyte motor activity.
- A signaling cascade involving chemokinetic input to aPKCs regulates T cell locomotion.
