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Related Experiment Video

Updated: Jul 10, 2026

The Corneal Micropocket Assay: A Model of Angiogenesis in the Mouse Eye
11:49

The Corneal Micropocket Assay: A Model of Angiogenesis in the Mouse Eye

Published on: August 16, 2014

The mouse cornea micropocket angiogenesis assay.

Michael S Rogers1, Amy E Birsner, Robert J D'Amato

  • 1The Vascular Biology Program, Children's Hospital Boston, and Department of Ophthalmology, Harvard Medical School, 300 Longwood Ave., Boston, Massachusetts 02115, USA.

Nature Protocols
|October 20, 2007
PubMed
Summary
This summary is machine-generated.

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The mouse corneal micropocket assay provides a reliable in vivo method to study blood vessel growth (angiogenesis). This assay uses slow-release pellets to stimulate and quantify predictable angiogenic responses in the cornea over five days.

Area of Science:

  • Ophthalmology
  • Vascular Biology
  • Biomedical Engineering

Background:

  • The cornea is an avascular tissue, making it an ideal model for studying angiogenesis in vivo.
  • Angiogenesis, or new blood vessel formation, is crucial in various physiological and pathological processes.

Purpose of the Study:

  • To describe and validate the mouse corneal micropocket assay for studying angiogenesis.
  • To establish a standardized method for assessing the angiogenic potential of molecules and genetic factors.

Main Methods:

  • Preparation of slow-release pellets by mixing angiogenic growth factors (e.g., VEGF, bFGF) with stabilizers (sucralfate) and polymers (Hydron).
  • Surgical implantation of pellets into a micropocket created in the avascular mouse cornea.
  • Quantification of the angiogenic response by measuring corneal neovascularization using a slit lamp after 5 days.

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Related Experiment Videos

Last Updated: Jul 10, 2026

The Corneal Micropocket Assay: A Model of Angiogenesis in the Mouse Eye
11:49

The Corneal Micropocket Assay: A Model of Angiogenesis in the Mouse Eye

Published on: August 16, 2014

A Mouse Model of the Cornea Pocket Assay for Angiogenesis Study
06:13

A Mouse Model of the Cornea Pocket Assay for Angiogenesis Study

Published on: August 18, 2011

An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis
06:10

An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis

Published on: August 6, 2020

Main Results:

  • The assay generates a predictable and quantifiable angiogenic response within 5 days.
  • A skilled investigator can process approximately 40 eyes in 2.5 hours, demonstrating efficiency.
  • The assay effectively assesses the ability of therapeutic molecules or genetic variations to modulate angiogenesis.

Conclusions:

  • The mouse corneal micropocket assay is a robust and efficient in vivo model for angiogenesis research.
  • This assay facilitates the evaluation of novel therapeutic strategies targeting angiogenesis.
  • The method allows for the assessment of genetic influences on blood vessel formation.