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Updated: May 9, 2026

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis
Published on: December 21, 2010
Reproducibility of two-dimensional gel electrophoresis at different replication levels
Cristina-Maria Valcu1, Mihai Valcu
1Technical University of Munich, Section of Forest Genetics, Freising, Germany. valcu@wzw.tum.de
Abstract:
Reliability of two-dimensional electrophoresis differential display experiments depends on the reproducibility of the separations. The contribution of biological and technical variation to the overall variance of the two-dimensional patterns was estimated based on the factors found to influence spot volume variance. The second dimension and the staining were responsible for most of the spot volume variance, while using pooled samples lowered biological variation to the level of technical variation.
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