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Enhanced background rejection in thick tissue with differential-aberration two-photon microscopy
1Boston University, Department of Biomedical Engineering, Boston, Massachusetts, USA.
Biophysical Journal
|October 24, 2007
Summary
We developed a differential aberration imaging technique to reduce background noise in two-photon excited fluorescence (TPEF) microscopy. This method effectively enhances imaging deep within thick tissues by selectively quenching in-focus signals.
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