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Analysis of TIMP expression and activity

Linda Troeberg, Hideaki Nagase

    Methods in Molecular Medicine
    |October 24, 2007
    PubMed

    This chapter provides practical information on the assay of tissue inhibitor of metalloproteinase (TIMP) activity and background information enabling meaningful interpretation of the data. Protocols are given for assessing the presence of TIMPs in biological samples by immunoblotting and by virtue of their ability to inhibit matrix metalloproteinase (MMP) hydrolysis of protein substrates (reverse zymography) and synthetic fluorogenic substrates.

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