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Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
Published on: October 31, 2015
Fluorescence microscopy to study domains in supported lipid bilayers.
Jonathan M Crane1, Lukas K Tamm
1Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, USA.
Methods in Molecular Biology (Clifton, N.J.)
|October 24, 2007
Summary
This study outlines techniques for creating complex model membranes using planar supported bilayers for fluorescence microscopy. These methods allow controlled lipid composition and asymmetry, aiding phase behavior analysis.
Area of Science:
- Biophysics
- Materials Science
- Cell Biology
Background:
- Fluorescence microscopy is vital for studying lipid mixtures in model membranes.
- Planar supported bilayers offer superior control over composition and asymmetry compared to spherical models.
- These systems allow easy access for perfusion of soluble components.
Purpose of the Study:
- To detail techniques for reconstituting complex lipid bilayers in planar systems.
- To describe methods for forming both symmetric and asymmetric bilayers.
- To discuss considerations for lipid selection, fluorescent dyes, and microscopy setups.
Main Methods:
- Reconstitution of complex lipid mixtures into planar supported bilayers.
- Formation of both symmetric and asymmetric lipid bilayers.
- Utilizing fluorescence microscopy for phase behavior analysis.
Main Results:
- Established effective methods for creating controlled lipid bilayers.
- Demonstrated the utility of planar systems for complex lipid mixtures.
- Provided guidance on selecting lipids, dyes, and microscopy parameters.
Conclusions:
- Planar supported bilayers are a versatile platform for studying lipid phase behavior.
- The outlined techniques facilitate detailed investigation of biologically relevant lipid mixtures.
- This work provides a framework for advanced model membrane research.
