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Updated: Jan 26, 2026

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
Published on: July 17, 2021
Monitoring and visualization of baculovirus infection using green fluorescent protein strategy
Hyung Joon Cha1, William E Bentley
1Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, Korea.
Abstract:
Through green fluorescent protein (GFP) strategy, facile and fast monitoring and visualization of baculovirus infection in insect cells is possible in vivo. This chapter describes two novel techniques for simple determination of virus titer in the baculovirus expression system using GFP coexpression and rapid monitoring of infection of Sf-9 insect cells using combination of GFP and early-to-late (ETL) promoter. It is anticipated that the use of GFP under the control of ETL promoter will facilitate vector construction, virus isolation, and titer determination.
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