Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

Small interfering RNAs (siRNA)

Small interfering RNAs (siRNA)

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mediated Electrochemical Probing and Machine Learning for Cysteine and Reduced Monoclonal Antibody Quantification.

Biotechnology and bioengineering·2026

Same author

Genetically-Programmed Hypervesiculation of Lactiplantibacillus Plantarum Increases Production of Bacterial Extracellular Vesicles with Therapeutic Efficacy in a Preclinical Inflammatory Bowel Disease Model.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025

Same author

Scrambling Signal Modularity in Bottom-up Assembled Synthetic Pseudomonas Consortia Reveals Robust Information Transfer.

ACS synthetic biology·2025

Same author

Electrochemistry as a Tool for Redox-Based Bio-Information Processing.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025

Same author

Assessing electrogenetic activation via a network model of biological signal propagation.

Frontiers in systems biology·2025

Same author

Genetically-programmed Hypervesiculation of <i>Lactiplantibacillus plantarum</i> Increases Production of Bacterial Extracellular Vesicles with Therapeutic Efficacy in a Preclinical Inflammatory Bowel Disease Model.

bioRxiv : the preprint server for biology·2025

Search research articles

Related Experiment Video

Updated: Jul 10, 2026

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Published on: December 28, 2016

Methods for gene silencing with RNAi.

John C March¹, William E Bentley

¹Department of Biological and Environmental Engineering, Cornell University, Ithaca, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)

|October 24, 2007

Summary

This study presents a rapid method for synthesizing and transfecting double-stranded RNA (dsRNA) in insect cell cultures, enabling efficient RNA interference. The protocol includes optimized dsRNA design strategies and takes only 1 hour for transfection with quick cell recovery.

More Related Videos

Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae

Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae

Published on: March 25, 2015

DNA Vector-based RNA Interference to Study Gene Function in Cancer

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

Related Experiment Videos

Last Updated: Jul 10, 2026

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Published on: December 28, 2016

Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae

Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae

Published on: March 25, 2015

DNA Vector-based RNA Interference to Study Gene Function in Cancer

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

Area of Science:

Molecular Biology
Cell Biology
Biotechnology

Background:

RNA interference (RNAi) is a powerful gene silencing technique.
Efficient delivery of double-stranded RNA (dsRNA) is crucial for RNAi applications in cell cultures.
Sf-21 insect cell line is a common model for studying gene function.

Purpose of the Study:

To describe a streamlined technique for synthesizing and transfecting dsRNA in Sf-21 cells.
To provide guidelines and resources for effective dsRNA design.
To optimize the RNAi process in insect cell culture.

Main Methods:

Synthesis of dsRNA using Ambion's MEGAscript T7 kit.
Transfection of Sf-21 cells with dsRNA.
Utilizing Ambion's RNAqueous-4PCR kit for RNA extraction.
Employing Invitrogen's Superscript III kit for cDNA synthesis.

Main Results:

A rapid dsRNA transfection protocol requiring only 1 hour.
Sf-21 cells typically recover within 12 hours post-transfection.
Included suggestions and online resources for designing effective dsRNA sequences.

Conclusions:

The described method offers an efficient and rapid approach for RNA interference in Sf-21 cell cultures.
Optimized dsRNA design is critical for successful gene silencing.
The protocol facilitates quick gene function studies in insect cells.