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Neuroprotective effect of mild hypothermia in the temporary brain ischemia in cats
Hiroshi Nakano1, Benedicto Oscar Colli, Luiza da Silva Lopes
1Division of Neurosurgery, Department of Surgery and Anatomy, Hospital das Clinicas, Ribeirão Preto Medical School, University of São Paulo, 14048-900 Ribeirão Preto, SP, Brazil.
Objective:
To evaluate the neuroprotective effect of mild hypothermia during temporary focal ischemia in cats.
Method:
20 cats underwent middle cerebral artery 60 minutes occlusion and 24 hours reperfusion: 10 under normothermia and 10 under mild hypothermia (32 masculine C). Brain coronal sections 2mm thick were stained with 2,3,5-triphenyltetrazolium hydrochloride, photographed and evaluated with software for volume calculation.
Results:
Cortical ischemia was found in 7 and basal ganglia ischemia in 8 animals of group 1 and in both regions in 5 animals of group 2 (no difference: p=0.6499 for cortical; p=0.3498 for basal ganglia). No ischemia was found in 5 animals of group 2 and in none of group 1 (significant difference, p=0.0325). The infarct volume was greater in group 1 than 2 (p=0.0433).
Conclusion:
Mild hypothermia did not interfere with location of ischemia, but it was effective for reducing the infarct volume.
