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High Throughput Screening Assessment of Reactive Oxygen Species (ROS) Generation using Dihydroethidium (DHE) Fluorescence Dye
Published on: January 19, 2024
[Effect of chitosan on the level of ROS in HepG2 cell induced by cadmium chloride]
Hong Xie1, Jiachun Wang, Guanyu Fan
1MOE Key Laboratory of Environment and Health, Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Objective:
To study the effect of chitosan on ROS levels in HepG2 cell induced by cadmium chloride.
Methods:
The experiments were divided into 6 groups: normal control group, cadmium chloride group (20micromol/L), chitosan group (0.50mg/ml) and three combined treatment group (cadmium chloride 20micromol/L and chitosan 0.02mg/ml, 0.10mg/ml and 0.50mg/ml). The HepG2 cells were treated by chitosan and cadmium chloride simultaneously for 4h. The fluorescence of 2', 7'-dichlorofluorescin (DCF) was measured by floweytometry as a mean of estimating the formation of reactive oxygen species (ROS).
Results:
In the comparison with normal control, the DCF intensity of chitosan group only exhibited somewhat decrease (P > 0.05) and significantly increased in cadmium chloride group (20micromol/L) (P < 0.01). The DCF intensity decreased in all three combined groups were more higher than those of cadmium chloride group, and when the concentration of chitosan improved from 0.01mg/ml to 0.50mg/ml, the DCF intensity decreased from 275.593 to 174.597 and the DCF intensity significant decrease was observed at concentration of 0.10mg/ml and 0.50mg/ml chitosan.
Conclusion:
It was suggested that chitosan could antagonize the increase of ROS in HepG2 cell induced by cadmium chloride.