[Blood toxicity assessment of subchronic exposure to hydroxylammonium nitrate in wistar rats ]
Hui An1, Lujun Yang, Yanhong Zhou
1Department of Military Toxicology, Preventive Medicine College, the Third Military Medical University, Chongqing 400038, China. anhui77@hotmail.com
Objective:
To investigate the effects of hydroxylammonium nitrate (HAN) on the blood system in rats.
Methods:
A total of 160 rats were randomly divided into three HAN treated groups and one negative control group, each group included 40 rats. Different doses of HAN at 6.97, 13.93 and 27.86mg/kg were administered to the three treated groups by intraperitoneal injection, and the control group was treated with 0.9% saline, the treatment had been performed every other day for 13 weeks. After the last treatment, 30 rats of each group were sacrificed and the rest were kept for another 4 weeks for recovery. Hematological parameters, reticulocyte and marrow cells were observed and measured.
Results:
For hematological parameters, the main changes were the decrease of RBC and HGB level and the increase of WBC at the end of the administration (P < 0.05). The reticulocyte values were obviously increase along with the increasing HAN dosage compared with those of control group (P < 0.01). For bone marrow cells observation, the ratio of erythrocytic line increased, and the ratio of granulocytes/erythrocyte was lower than that of control group (P < 0.05). After 4 weeks' recovery period, no obviously changes were observed for hematological parameters, reticulocyte and marrow cells.
Conclusion:
HAN has toxic effect on the blood system in rats for long-term exposure.
