Related Experiment Video
Updated: Jul 10, 2026

Evaluation of the Spatial Distribution of γH2AX following Ionizing Radiation
Published on: August 7, 2010
[Association between gamma-H2AX and DNA double-strand breaks]
1National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Abstract:
H2AX may be one family of histone and distributes among the genome. It is reported that DNA double-strand breaks (DSB) could induce phosphorylation of H2AX at conservative C-terminal region of serine 139 and the formation of gamma-H2AX. The gamma-H2AX foci can be observed by the fluorescence microscope. An immunofluorescence assay with specific antibodies recognizing gamma-H2AX could become the gold standard for detecting the DSB. It was indicated that some of physical, chemical, and biological factors could induce the formation of the gamma-H2AX foci. In this paper, the advance of the studies of mechanism of gamma-H2AX formation and the association between gamma-H2AX and DSB were reviewed.
Related Concept Videos
Fixing Double-strand Breaks
Fixing Double-strand Breaks
Homologous Recombination
DNA Damage can Stall the Cell Cycle
DNA Damage Can Stall the Cell Cycle
Nucleotide Excision Repair
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...

