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Updated: Jul 10, 2026
Analysis of Developing Tooth Germ Innervation Using Microfluidic Co-culture Devices
Published on: August 14, 2015
[Expressions of fibronectin and bone morphogenetic protein-2, 4 during development of mouse tooth germ]
Jian Zhang1, Wei-dong Tian, Xin Nie
1Department of Oral and Maxillofacial Surgery, West China Stomatological Hospital, Sichuan University, Chengdu 610041, China.
Objective:
The purpose of this study was to investigate the distribution and relationship of fibronectin (FN), bone morphogenetic protein (BMP) 2, 4 during development of mouse tooth germ.
Methods:
Rat embryonic first molars were collected from E14 (bud stage) and E18 (bell stage). The expressions of FN and BMP-2, 4 were analyzed with immunohistochemistry.
Results:
FN was located in the epithelium and dental mesenchyme on bud stage, but on bell stage, the FN was found at the region of differentiating odontoblasts and in the inner enamel epithelium, and also BMP-2, 4 were abundant mainly at the brisk region of differentiating odontoblasts.
Conclusion:
The results demonstrate that BMP-2, 4 and FN play the important roles during bud stage and bell stage, and there may be synergy between BMP-2, 4 and FN in regulating differentiation and maturity of odontoblasts.
