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Updated: Jul 10, 2026

Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates
Published on: April 22, 2019
[Expression and action of keratinocyte growth factor, keratinocyte growth factor receptor on CaSki cell]
1Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To investigate the expressions of keratinocyte growth factor (KGF), keratinocyte growth factor receptor (KGFR) in CaSki cell and action of keratinocyte growth factor on proliferation, migration of the CaSki cell.
Methods:
ELISA and Western blot methods were used to determine the protein expressions of the KGF and KGFR in CaSki cell respectively: 3H-Thymidine incorporation method was used to determine the effect of recombinant human KGF and anti-KGF on CaSki cell proliferation; Millicell-PCF was used to determine the effect of recombinant human KGF, anti-KGF on CaSki cell migration.
Results:
Both KGF and KGFR were expressing in the CaSki cell; Recombinant human KGF resulted in a increase in the proliferation and migration of CaSki cells; The proliferation and migration of CaSki cell became weak due to autocrine KGF neutralized by KGF antibodies (P < 0.05).
Conclusion:
Current results demonstrate that KGF and KGFR express in CaSki cell; Both autocrine and recombinant human KGF have the effect on CaSki cell proliferation and migration.
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