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Updated: Jul 10, 2026
Quantitative Analysis of Dietary Vitamin A Metabolites in Murine Ocular and Non-Ocular Tissues Using High-Performance Liquid Chromatography
Published on: December 27, 2024
[Detecting VA, VD3, VE and beta-carotene with HPLC in human serums]
Xiao-li Zou1, Hong-yan Zeng, Yuan-qian Li
1Department of Sanitary Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Objective:
To develop a method of detecting retinol (vitamin A), vitamin D3, alpha-tocopherol (vitamin E) and beta-carotene in human serums with HPLC.
Methods:
Proteins were precipitated with anhydrous ethanol. Fat-solutable vitamins in human serums were extracted with aether/petroleum ether mixture and determined with HPLC.
Results:
The linear ranges for the retinol, VD3, alpha-tocopherol and beta-carotene were 0.012 microg/mL-500 microg/mL, 0.030 microg/mL-500 microg/mL, 0.12 microg/mL-500 microg/mL, and 0.015 microg/mL-500 microg/mL, respectively. The detection limits for the retinol, VD3, alpha-tocopherol and beta-carotene were 0.012 microg/mL, 0.030 microg/mL, 0.12 microg/mL and 0.015 microg/mL, respectively. The relative standard derivations (RSD) for the retinol, VD3, alpha-tocopherol and beta-carotene were 0.75%, 0.54%, 2.06% and 2.74%, respectively. The proposed method recovered 92%-116%, 98%-112%, 84.8%-106%, and 90%-105% retinol, VD3, alpha-tocopherol and beta-carotene in human serums respectively.
Conclusion:
The method is simple, quick and applicable to all of the four fat-solutable vitamins.