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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Rapid Identification of Pathogens01:25

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...

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Related Experiment Video

Updated: Jul 10, 2026

Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
07:49

Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa

Published on: September 9, 2013

LC-MALDI MS and MS/MS--an efficient tool in proteome analysis.

Dieter R Mueller1, Hans Voshol, Annick Waldt

  • 1Novartis Institutes for BioMedical Research, Genome and Proteome Sciences Systems Biology, Basel, Switzerland. dieter.mueller@novartis.com

Sub-Cellular Biochemistry
|October 24, 2007
PubMed
Summary

Liquid chromatography-matrix-assisted laser desorption/ionization mass spectrometry (LC-MALDI MS) is a versatile technique for proteome analysis. Its flexibility, especially in off-line mode, allows for optimized separation and sensitive detection of peptides, even in complex samples.

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Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
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Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Mass Spectrometry

Background:

  • Liquid chromatography-matrix-assisted laser desorption/ionization mass spectrometry (LC-MALDI MS) is a powerful hyphenated technique for proteome analysis.
  • Various interfaces exist for coupling liquid chromatography (LC) with MALDI MS, each with distinct properties and performance characteristics.

Purpose of the Study:

  • To discuss on-line and off-line LC-MALDI MS techniques, focusing on their properties and performance.
  • To highlight the advantages of LC-MALDI MS, particularly in off-line mode, for peptide mixture analysis.
  • To present selected applications of LC-MALDI MS in proteome analysis.

Main Methods:

  • Discussion of on-line and off-line LC-MALDI MS configurations.
  • Optimization of chromatographic separation and mass spectrometry (MS/MS) parameters.
  • Application of labeling techniques for quantitative analysis.
  • Analysis of peptide mixtures and protein samples.

Main Results:

  • Off-line LC-MALDI MS offers flexibility by decoupling separation and analysis for individual optimization.
  • The technique is suitable for low to medium complexity peptide mixtures and can handle highly complex samples using multiplexing.
  • Quantitative analysis is feasible at both MS and MS/MS levels, aided by MALDI's ion formation characteristics.
  • LC-MALDI MS demonstrates flexibility and sensitivity in applications like membrane protein analysis and protein interaction studies.

Conclusions:

  • LC-MALDI MS, especially in off-line mode, is a flexible and sensitive technique for diverse proteome analysis workflows.
  • The ability to optimize parameters independently enhances its applicability for various sample complexities.
  • LC-MALDI MS complements other techniques like LC-ESI MS and is valuable for specific applications.