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Novel arylhydrazone-conjugated gold nanoparticles with DNA-cleaving ability: the first DNA-nicking nanomaterial

Ming-Hua Hsu1, Thainashmuthu Josephrajan, Chen-Sheng Yeh

  • 1Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan, ROC.

Bioconjugate Chemistry
|October 24, 2007
PubMed

Abstract:

Arylhydrazones were linked onto gold nanoparticles through the poly(ethylene glycol) spacer to produce a new type of photoinduced DNA-cleaving nanomaterials with great potency.

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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