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Updated: Jul 10, 2026
11:46
Shotgun Lipidomics of Rodent Tissues
Published on: November 18, 2022
Shotgun lipidomics by tandem mass spectrometry under data-dependent acquisition control
Dominik Schwudke1, Gerhard Liebisch, Ronny Herzog
1MPI of Molecular Cell Biology and Genetics, Dresden, Germany.
Methods in Enzymology
|October 24, 2007
Summary
This study introduces LipidInspector software for lipidomics, enabling comprehensive structural characterization of lipid precursors through data-dependent acquisition and Boolean scans. It enhances lipid analysis specificity and sensitivity, particularly with high-resolution mass spectrometers.
Area of Science:
- Lipidomics
- Mass Spectrometry
- Computational Biology
Background:
- Lipidomics research generates complex datasets requiring advanced interpretation tools.
- Current methods for lipid precursor analysis can be limited in specificity and sensitivity.
Purpose of the Study:
- To present LipidInspector software for in-depth structural characterization of lipid precursors.
- To demonstrate the utility of data-dependent acquisition and emulated scans for lipid analysis.
Main Methods:
- Utilized data-dependent acquisition of full MS/MS spectra from lipid precursors.
- Employed LipidInspector software for data interpretation, including Boolean scans.
- Emulated selected reaction monitoring (SRM) analysis for targeted lipid detection.
Main Results:
- LipidInspector enables the emulation of unlimited precursor and neutral loss scans.
- Boolean scans allow for highly specific data interpretation and structural characterization.
- Emulated SRM analysis achieved high detection specificity and sensitivity for preselected lipids.
Conclusions:
- Data-dependent acquisition coupled with LipidInspector offers a powerful approach for lipidomics.
- The software facilitates comprehensive lipid structural elucidation and targeted analysis.
- High-resolution mass spectrometry, especially Orbitrap and FT-ICR, significantly benefits this approach.
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