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Updated: Jul 10, 2026
12:26
Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
Published on: May 3, 2018
Ceramide kinase and ceramide-1-phosphate.
Dayanjan S Wijesinghe1, Nadia F Lamour, Antonio Gomez-Munoz
1Department of Biochemistry and Molecular Biology, Virginia Commonwealth University School of Medicine, Richmond, Virginia, USA.
Methods in Enzymology
|October 24, 2007
Summary
Ceramide-1-phosphate (C1P), a sphingolipid, regulates distinct biological mechanisms via ceramide kinase (CERK). This chapter details methods to study CERK function and C1P
Area of Science:
- Biochemistry
- Cell Biology
- Lipid Metabolism
Background:
- Sphingolipid ceramide-1-phosphate (C1P) has been known for over a decade.
- Recent research reveals diverse biological functions regulated by C1P.
- Ceramide kinase (CERK) is identified as a key enzyme in C1P production.
Purpose of the Study:
- To provide detailed methodologies for studying ceramide kinase (CERK) function.
- To outline methods for assessing ceramide-1-phosphate (C1P) levels.
- To describe techniques for observing C1P's specific biological effects using exogenous C1P.
Main Methods:
- In vitro assays for examining ceramide kinase (CERK) activity.
- Cell-based experiments to investigate CERK function and C1P production.
- Quantification of C1P levels in biological samples.
- Application of exogenous C1P to cellular models to determine lipid-specific effects.
Main Results:
- Established methodologies enable robust investigation of CERK and C1P.
- Detailed protocols facilitate reproducible research in lipid signaling.
- The presented methods allow for the elucidation of C1P-mediated biological processes.
Conclusions:
- Comprehensive methodologies are now available for studying ceramide kinase (CERK) and ceramide-1-phosphate (C1P).
- These methods will advance the understanding of C1P's role in various biological mechanisms.
- Further research into C1P signaling pathways is facilitated by these detailed experimental approaches.
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