Related Experiment Video
Updated: Jul 10, 2026
The Establishment of a Murine Mandibular Molar Extraction Socket Healing Model
Published on: January 13, 2023
Ovariectomy delays alveolar wound healing after molar extractions in rats
Michele Conceição Pereira1, Karina Gottardello Zecchin, Eduardo Bauml Campagnoli
1Department of Oral Diagnosis, Dental School, State University of Campinas (UNICAMP), Piracicaba, São Paulo, Brazil.
Purpose:
This study was conducted to investigate the morphological effects of the absence of estrogen on alveolar wound healing of young female rats after tooth extraction.
Materials And Methods:
A total of 60 4- to 6-week-old female rats underwent bilateral ovariectomy (OVX) or sham operations. Three weeks later, the first mandibular molars were extracted. Subsequently, the animals were killed by cervical dislocation 3, 5, 7, 14, 21, or 28 days after tooth extraction. The mandibles were removed, and serial transversal sections of mesial alveolus of the first mandibular molars were obtained for histometric analysis.
Results:
OVX sockets showed significant increases in fibroblasts and collagen content 3 and 5 days after the extractions, followed by significant decreases in these parameters in the subsequent periods. In accordance with the decreased collagen content in the latest period of healing, new bone formation was significantly reduced in the OVX animals.
Conclusion:
These findings suggest that the initial molecular changes observed in the absence of estrogen lead to delayed alveolar wound healing.
