[Phenotype changes in smooth muscle cells of human coronary arteries during aging and during development of atherosclerosis]

The ultrastructure and the expression of cytoskeletal and contractile proteins were studied in the intimal cells of human coronary arteries (CA) taken at autopsy from 38 trauma victims aged 1 to 70 years. All intimal smooth muscle cells (SMC) of the CA from 2-4-year old children contained desmin, vimentin, myosin, and actin. In the normal intima of adolescents aged 14-16 years, only did some SMC contain desmin whereas in that of adults, they had no desmin, but expressed all other proteins. For example, some atherosclerotic plaques of CA exhibited desmin-positive SMC and smooth muscle myosin-free cells. The ultrastructure of SMC of atherosclerotic plaques showed profound polymorphism. In addition to typical SMC, the plaques displayed modified cells having a developed endoplasmic reticulum and Golgi complex. The fact that the atherosclerotic plaques have cells differing in ultrastructural features and protein expression, which is specific to an earlier period of the body development suggests phenotypic changes in the cells and the latter acquiring new functions that are of great significance in the pathogenesis of atherosclerosis.