Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Formation of the Platelet Plug

Formation of the Platelet Plug

The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...

Structure and Function of Platelets

Structure and Function of Platelets

The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000 platelets, with...

Inflammation

Inflammation

Acute Inflammation I: Inflammatory Response

Acute Inflammation I: Inflammatory Response

Acute inflammation is a rapid, short-lived physiological response to tissue injury or infection, designed to eliminate harmful agents and initiate repair. This tightly regulated process typically lasts from minutes to several days and is triggered by factors such as microbial invasion, physical trauma, or chemical injury.Recognition and Mediator ReleaseThe inflammatory response begins when resident immune cells—such as mast cells, macrophages, and dendritic cells—detect damage-associated...

Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors

Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors

Antiplatelet drugs emerge as frontline defenders against the insidious threat of thromboembolic diseases, where abnormal clots obstruct vital blood vessels. These drugs stand as bulwarks, inhibiting platelet aggregation and clot formation, thereby mitigating the risk of life-threatening conditions like myocardial infarction, coronary artery disease, and thrombotic strokes.
Prostaglandin synthesis inhibitors, exemplified by the widely known aspirin, wield their power by irreversibly acetylating...

Acute Inflammation II: Cellular Phase

Acute Inflammation II: Cellular Phase

The cellular phase of acute inflammation is a tightly orchestrated sequence of events that recruits leukocytes, primarily neutrophils, to sites of tissue injury or infection. Following the initial vascular changes, this phase ensures effective immune cell migration, activation, and function at the affected site to eliminate pathogens and initiate tissue repair.Leukocyte Recruitment CascadeLeukocyte recruitment happens in four steps: margination, adhesion, transmigration, and chemotaxis. Reduced...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A novel murine model of pulmonary fibrosis: the role of platelets in chronic changes induced by bleomycin.

Journal of pharmacological and toxicological methods·2021

Same author

Radiolabelling and immunohistochemistry reveal platelet recruitment into lungs and platelet migration into airspaces following LPS inhalation in mice.

Journal of pharmacological and toxicological methods·2019

Same author

Use of animal models in IPF research.

Pulmonary pharmacology & therapeutics·2018

Same author

Lipopolysaccharide (LPS) induced pulmonary neutrophil recruitment and platelet activation is mediated via the P2Y<sub>1</sub> and P2Y<sub>14</sub> receptors in mice.

Pulmonary pharmacology & therapeutics·2017

Same author

Novel uses for anti-platelet agents as anti-inflammatory drugs.

British journal of pharmacology·2007

Same author

Platelet activation in asthma: integral to the inflammatory response.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2006

Search research articles

Related Experiment Video

Updated: Jul 10, 2026

An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers

An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers

Published on: November 8, 2024

Defining a role for platelets in allergic inflammation.

S C Pitchford¹

¹Leukocyte Biology Section, National Heart and Lung Institute, Imperial College, London SW7 2AZ, U.K. s.pitchford@imperial.ac.uk

Biochemical Society Transactions

|October 25, 2007

Summary

Platelets act as inflammatory cells, distinct from their role in blood clotting. Their involvement in inflammation and tissue remodeling suggests potential therapeutic targets for asthma and allergies.

Area of Science:

Immunology
Hematology
Pulmonology

Background:

Platelets, traditionally known for thrombosis and hemostasis, are increasingly recognized for their inflammatory roles.
Chronic asthma involves airway inflammation, hyper-responsiveness, and tissue remodeling, with observed platelet function changes in allergic diseases.
Platelets express adhesion molecules, activate leukocytes, release inflammatory mediators, and can migrate, indicating a broader role in immune responses.

Purpose of the Study:

To explore the multifaceted role of platelets in inflammatory processes, particularly in the context of asthma and allergic diseases.
To investigate platelet involvement in airway inflammation, hyper-responsiveness, and tissue remodeling.
To assess the therapeutic potential of targeting platelet activity in allergic conditions.

More Related Videos

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Published on: June 10, 2025

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice

Published on: April 2, 2013

Related Experiment Videos

Last Updated: Jul 10, 2026

An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers

An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers

Published on: November 8, 2024

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Published on: June 10, 2025

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice

Published on: April 2, 2013

Main Methods:

Review of clinical data from asthma, allergic rhinitis, and dermatitis patients showing altered platelet behavior.
Utilizing mouse models of allergic inflammation to study platelet-dependent recruitment of eosinophils and lymphocytes.
Employing models of chronic inflammation to evaluate platelet participation in tissue remodeling, comparing platelet depletion with glucocorticosteroid treatment.

Main Results:

Platelet activation and function changes are observed in patients with allergic diseases.
Platelets, via P-selectin, are crucial for eosinophil and lymphocyte recruitment to the lungs in allergic inflammation models.
Platelet depletion is more effective than glucocorticosteroids in suppressing airway remodeling in chronic inflammation models.
Platelets demonstrate chemotaxis and migration capabilities within inflamed tissues, localizing to specific sites.
Platelet activation is linked to both innate and adaptive immune responses, influencing pathophysiological events.

Conclusions:

Platelets possess significant inflammatory functions beyond hemostasis, contributing to allergic airway inflammation and tissue remodeling.
Platelet activity plays a critical role in asthma pathogenesis, including airway hyper-responsiveness and structural changes.
Targeting platelet function represents a promising therapeutic strategy for managing asthma and other allergic conditions.