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Related Concept Videos

Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

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Related Experiment Video

Updated: Jul 10, 2026

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model
10:23

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Published on: November 13, 2012

Mouse models for BRAF-induced cancers.

C Pritchard1, L Carragher, V Aldridge

  • 1Department of Biochemistry, Henry Wellcome Building, University of Leicester, Lancaster Road, Leicester LE1 7RH, U.K. cap8@le.ac.uk

Biochemical Society Transactions
|October 25, 2007
PubMed
Summary
This summary is machine-generated.

Oncogenic BRAF mutations, especially V600E, drive cancer by promoting cell proliferation. Genetically engineered mouse models show BRAF inhibition can reverse cancer hallmarks, highlighting therapeutic potential.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Oncogenic BRAF mutations are frequent in human cancers, particularly melanoma.
  • The V600E mutation is the most common BRAF alteration, driving cancer hallmarks like proliferation and survival.
  • BRAF and MEK are validated therapeutic targets for BRAF-mutated cancers.

Purpose of the Study:

  • To understand the in vivo role of oncogenic BRAF in cancer development.
  • To evaluate the efficacy of anti-BRAF or anti-MEK therapies in vivo.
  • To utilize genetically engineered mouse models (GEMMs) to study BRAF-driven tumorigenesis.

Main Methods:

  • Generation of GEMMs with Cre-lox based conditional expression of oncogenic BRAF.
  • Temporal and spatial control of Cre recombinase delivery for BRAF activation in specific tissues.
  • Analysis of tumor initiation, proliferation, and senescence following BRAF mutation induction.

Main Results:

  • (V600E)BRAF mutation drives tumor initiation in hematopoietic and lung tissues.
  • BRAF-driven tumorigenesis is primarily mediated by cyclin D1-induced cell proliferation.
  • Oncogene-induced senescence (OIS) acts as a barrier to tumor progression.

Conclusions:

  • GEMMs are valuable tools for studying BRAF-driven cancers and testing therapies in vivo.
  • BRAF and MEK are critical targets for cancer therapy.
  • Understanding the interplay between BRAF, proliferation, and senescence is key for effective cancer treatment.