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[Plasmid analysis of antibiotic resistant Shigella isolates]
G Ozcengiz1, M McMenamin, L B Quesnel
1Orta Doğu Teknik Universitesi Fen ve Edebiyat Fakultesi Biyoloji Bölümü, Ankara.
Mikrobiyoloji Bulteni
|October 1, 1991
Summary
This study investigated the link between drug resistance and plasmid profiles in Shigella bacteria. Findings reveal a correlation, aiding in understanding antimicrobial resistance mechanisms.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Multiple drug resistance (MDR) in bacterial pathogens poses a significant public health threat.
- Plasmids are extrachromosomal DNA elements frequently associated with the spread of antibiotic resistance genes.
- Shigella species are common causes of bacterial dysentery, and understanding their resistance mechanisms is crucial.
Purpose of the Study:
- To investigate the correlation between multiple drug resistance (MDR) patterns and plasmid profiles in clinical isolates of Shigella sonnei and Shigella flexneri.
- To characterize the plasmids harbored by these resistant Shigella strains.
Main Methods:
- Seventy clinical isolates of Shigella sonnei and Shigella flexneri were analyzed.
- Plasmid DNA was extracted from isolates.
- Plasmid profiles were determined.
- The most common plasmids were purified using electroelution.
- Purified plasmids were characterized by restriction endonuclease digestion.
Main Results:
- A correlation was identified between specific multiple drug resistance patterns and distinct plasmid profiles in the studied Shigella isolates.
- Characterization of common plasmids provided insights into their genetic makeup and potential role in conferring resistance.
Conclusions:
- Plasmid profiles are associated with the multiple drug resistance patterns observed in Shigella sonnei and Shigella flexneri.
- Understanding these plasmid-mediated resistance mechanisms is essential for developing effective treatment strategies against Shigella infections.