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[Plasmid analysis of antibiotic resistant Shigella isolates]
G Ozcengiz1, M McMenamin, L B Quesnel
1Orta Doğu Teknik Universitesi Fen ve Edebiyat Fakultesi Biyoloji Bölümü, Ankara.
Mikrobiyoloji Bulteni
|October 1, 1991
Abstract:
A correlation between the multiple drug resistance patterns and the plasmid profiles given by 70 clinical isolates of Shigella sonnei and Shigella flexneri was investigated in this study. The most common plasmids were purified from different isolates by electroelution and characterized via restriction endonuclease digestions.
Insights
This study investigated the link between drug resistance and plasmid profiles in Shigella bacteria. Findings reveal a correlation, aiding in understanding antimicrobial resistance mechanisms.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Multiple drug resistance (MDR) in bacterial pathogens poses a significant public health threat.
- Plasmids are extrachromosomal DNA elements frequently associated with the spread of antibiotic resistance genes.
- Shigella species are common causes of bacterial dysentery, and understanding their resistance mechanisms is crucial.
Purpose of the Study:
- To investigate the correlation between multiple drug resistance (MDR) patterns and plasmid profiles in clinical isolates of Shigella sonnei and Shigella flexneri.
- To characterize the plasmids harbored by these resistant Shigella strains.
Main Methods:
- Seventy clinical isolates of Shigella sonnei and Shigella flexneri were analyzed.
- Plasmid DNA was extracted from isolates.
- Plasmid profiles were determined.
- The most common plasmids were purified using electroelution.
- Purified plasmids were characterized by restriction endonuclease digestion.
Main Results:
- A correlation was identified between specific multiple drug resistance patterns and distinct plasmid profiles in the studied Shigella isolates.
- Characterization of common plasmids provided insights into their genetic makeup and potential role in conferring resistance.
Conclusions:
- Plasmid profiles are associated with the multiple drug resistance patterns observed in Shigella sonnei and Shigella flexneri.
- Understanding these plasmid-mediated resistance mechanisms is essential for developing effective treatment strategies against Shigella infections.