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Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube (SWCNT)-delivered MALAT1 Antisense Oligos
Published on: December 13, 2018
Single-walled carbon nanotube interactions with HeLa cells
Hadi N Yehia1, Rockford K Draper, Carole Mikoryak
1Department of Chemistry, The University of Texas at Dallas, Richardson, TX 75080, USA. pantano@utdallas.edu.
Journal of Nanobiotechnology
|October 25, 2007
Summary
Single-walled carbon nanotubes (SWNTs) were not cytotoxic to human epithelial-like HeLa cells in vitro. This study provides recommendations for accurate carbon nanotube (CNT) cytotoxicity assessments.
Area of Science:
- Biomaterials Science
- Cell Biology
- Nanotechnology
Background:
- Carbon nanotubes (CNTs) are increasingly explored for biomedical applications.
- Assessing the cytotoxicity of CNTs is crucial for their safe use.
- Standardized protocols for CNT cytotoxicity testing are needed.
Purpose of the Study:
- To evaluate the cytotoxicity of CoMoCAT single-walled carbon nanotubes (SWNTs) on HeLa cells.
- To investigate the cellular uptake and intracellular localization of SWNTs.
- To provide recommendations for improving CNT cytotoxicity studies.
Main Methods:
- Characterization of SWNTs using SEM, TGA, AFM, ICP-MS, and spectroscopy.
- Confocal microRaman spectroscopy for cellular uptake analysis.
- TEM for intracellular localization, cell morphology, growth rate assays, and flow cytometry for mitochondrial superoxide detection.
Main Results:
- SWNTs were internalized by HeLa cells in a time- and temperature-dependent manner.
- SWNTs were found within intracellular vacuoles.
- No significant differences in cell morphology, growth rates, or mitochondrial superoxide levels were observed between SWNT-exposed and control cells.
Conclusions:
- CoMoCAT SWNT dispersions, under the tested conditions, are not inherently cytotoxic to HeLa cells.
- The study highlights the importance of sample preparation and assay conditions in CNT cytotoxicity evaluations.
- Recommendations are provided for enhancing the accuracy and comparability of future CNT cytotoxicity reports.
