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Raman Spectroscopy Instrumentation: Overview

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Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

Multiplexed microfluidic surface-enhanced Raman spectroscopy.

Nahla A Abu-Hatab¹, Joshy F John, Jenny M Oran

¹University of Tennessee-Knoxville, Department of Chemistry, 552 Buehler Hall, Knoxville, Tennessee 37996-1600, USA.

Applied Spectroscopy

|October 26, 2007

Summary

Multiplexed microfluidics (MMFs) offer a novel, high-throughput method for sensitive and reproducible surface-enhanced Raman spectroscopy (SERS) detection. This approach enables robust analysis of analytes, including pesticides, with improved performance.

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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

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Published on: March 20, 2015

Area of Science:

Analytical Chemistry
Spectroscopy
Nanotechnology

Background:

Surface-enhanced Raman spectroscopy (SERS) is a powerful analytical technique for trace-level detection in chemical and biological applications.
Developing sensitive, reproducible, and robust SERS substrates remains a significant challenge.
Existing methods often face limitations in throughput and ease of implementation.

Purpose of the Study:

To introduce a novel method for SERS using multiplexed microfluidics (MMFs) in a polydimethylsiloxane platform.
To achieve parallel, high-throughput, and sensitive detection and identification of analytes.
To demonstrate the efficiency and applicability of the MMF-SERS approach for various analytes and SERS conditions.

Main Methods:

Utilized a polydimethylsiloxane-based microfluidic platform for multiplexed SERS analysis.
Employed a passive pumping method for delivering silver (Ag) colloids and analytes under flowing conditions.
Integrated a high numerical aperture microscope objective with a confocal-based Raman spectrometer for enhanced sensitivity.

Main Results:

Achieved SERS signal reproducibility better than 7% under flowing conditions.
Demonstrated high sensitivity, with detection limits down to low nM for crystal violet and sub-pM for Mitoxantrone.
Successfully obtained SERS spectra of various pesticides and evaluated the effects of anion activators and colloid types.

Conclusions:

The MMF-SERS approach provides a sensitive, reproducible, and robust platform for high-throughput analyte detection.
This method overcomes alignment issues and allows for facile manipulation of experimental conditions.
The developed platform is versatile for analyzing diverse analytes and optimizing SERS performance, including the use of novel silver nanoparticle morphologies.