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Corneal protein nitration in experimental uveitis
Mutay Aslan1, Iclal Yucel, Akif Ciftcioglu
1Department of Biochemistry, Akdeniz University School of Medicine, 07070 Antalya, Turkey. mutayaslan@akdeniz.edu.tr
Experimental Biology and Medicine (Maywood, N.J.)
|October 26, 2007
Summary
Inducible nitric oxide synthase (NOS-2) and protein nitration increase in the cornea during uveitis, contributing to tissue damage. Inhibiting NOS-2 may help manage uveitis complications.
Area of Science:
- Ophthalmology
- Immunology
- Biochemistry
Background:
- Inflammatory diseases like uveitis show increased inducible nitric oxide synthase (NOS-2).
- NOS-2 is implicated in pathological processes during inflammation.
Purpose of the Study:
- To evaluate corneal expression of NOS-2.
- To assess corneal protein nitration in a rat model of uveitis.
Main Methods:
- Uveitis induced via intravitreal lipopolysaccharide injection in rats.
- Corneal NOS-2 and nitrotyrosine (NO(2)Tyr) assessed by immunohistochemistry and Western blot.
- Vitreous nitrate/nitrite levels measured using Griess reagent.
Main Results:
- Increased corneal NOS-2 and NO(2)Tyr immunoreactivity observed in uveitis.
- Multiple nitrated protein bands detected in corneas of uveitic rats.
- Significantly higher vitreous nitric oxide levels in uveitis models.
Conclusions:
- Extensive protein nitration and reactive nitrogen species in the cornea contribute to uveitis tissue destruction.
- Selective NOS-2 inhibition could prevent uveitis complications and improve management.
