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Late-bolting transgenic Chinese cabbage obtained by RNA interference technique
Guang-Qing Xia1, Jun-Yi Zhu, Qi-Wei He
1Biology Department, Tonghua Normal University, Tonghua, Jilin 134001, China.
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao = Journal of Plant Physiology and Molecular Biology
|October 26, 2007
Summary
Inhibiting the LEAFY (LFY) gene in Chinese cabbage using RNAi delays flowering and bolting. This genetic approach creates plants with more branches and leaves, offering a resource for breeding late-bolting varieties.
Area of Science:
- Plant molecular biology
- Genetics
- Agricultural science
Background:
- The LEAFY (LFY) gene is crucial for regulating plant flowering time and phase transition.
- Under-expression of LFY in Arabidopsis leads to late flowering and increased branching.
Purpose of the Study:
- To investigate the effect of down-regulating LFY gene expression on delaying bolting and flowering in Chinese cabbage (Brassica rapa L. ssp. pekinensis) using RNA interference (RNAi).
Main Methods:
- RNA interference (RNAi) approach was employed to down-regulate LFY gene expression.
- Quantitative Reverse Transcription Polymerase Chain Reaction (RQ-RT-PCR) was used to analyze LFY gene expression levels in transgenic plants.
Main Results:
- Transgenic Chinese cabbage plants exhibited a delayed transition to the reproductive phase.
- Inhibition of LFY gene expression resulted in plants with more branches, more leaves, and reduced height.
- RQ-RT-PCR confirmed significantly reduced LFY gene expression in the transgenic plants.
Conclusions:
- RNAi-mediated inhibition of LFY gene expression effectively delays bolting in Chinese cabbage under cold-sensitive long-day conditions.
- The late-flowering Chinese cabbage generated can serve as a valuable genetic resource for breeding late-bolting varieties.
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