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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...

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Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
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Published on: April 17, 2012

Polymeric integrated selective enrichment target (ISET) for solid-phase-based sample preparation in MALDI-TOF MS.

Simon Ekström1, Lars Wallman, Göran Helldin

  • 1Department of Electrical Measurements, Lund Institute of Technology, S-221 00, Sweden. simon.ekstrom@elmat.lth.se

Journal of Mass Spectrometry : JMS
|October 26, 2007
PubMed
Summary

A novel polymer device, the Integrated Selective Enrichment Target (ISET), simplifies proteomic sample preparation. It enables efficient protein purification and concentration for matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS).

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Area of Science:

  • Polymer microfabrication
  • Proteomics
  • Mass Spectrometry

Background:

  • Traditional proteomic sample preparation can be complex and time-consuming.
  • Matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) requires high-quality samples for accurate analysis.

Purpose of the Study:

  • To report a polymer microfabricated device for integrated proteomic sample preparation and MALDI MS sample presentation.
  • To demonstrate the utility of the Integrated Selective Enrichment Target (ISET) for purification and concentration of proteins/peptides.

Main Methods:

  • Microfabrication of a polymer device (ISET) with perforated nanovials.
  • Utilizing selective extraction media (microbeads) within nanovials for purification.
  • Investigating the impact of surface roughness and material conductivity (polyetheretherketone - PEEK) on MALDI MS performance.

Main Results:

  • Successful fabrication of the ISET device.
  • Demonstration of solid-phase extraction and phosphopeptide capture using the ISET.
  • Characterization of challenges related to surface roughness and PEEK conductivity for MALDI MS.

Conclusions:

  • The ISET offers a disposable polymeric solution for streamlined proteomic sample preparation.
  • The device facilitates efficient protein/peptide enrichment prior to MALDI MS analysis.
  • Addressing material properties is crucial for optimizing MALDI MS readout with polymer-based devices.