Analysis of microcystins by capillary high performance liquid chromatography using a polymethacrylate-based...

Area of Science:

• Analytical Chemistry
• Environmental Science

Background:

• Microcystins (MCs) are toxic cyanobacterial peptides posing risks to aquatic ecosystems and human health.
• Accurate and efficient monitoring of MCs in water samples is crucial for public health and environmental safety.

Purpose of the Study:

• To develop and evaluate a polymethacrylate-based monolithic column for the capillary high-performance liquid chromatography (capillary-HPLC) separation of similar microcystins.
• To investigate the factors affecting separation performance and assess the method's suitability for real-world water sample analysis.

Main Methods:

• Preparation of a polymethacrylate-based monolithic column with dual hydrophobic and cation-exchange properties.
• Capillary-HPLC separation using Tris(hydroxymethyl)aminomethane buffer and acetonitrile as mobile phases with ultraviolet detection.
• Solid-phase extraction for sample cleanup and preconcentration of microcystins from spiked lake water.

Main Results:

• Baseline separation of three microcystin variants (MC-LR, MC-YR, MC-RR) achieved in under 5 minutes.
• Linear calibration curves with high correlation coefficients (r>0.9990) over a concentration range of 0.25-18.00 mg/L.
• Low limits of detection (LODs) for MC-LR, MC-YR, and MC-RR (0.49, 0.67, 0.30 µg/L, respectively) in spiked lake water.

Conclusions:

• The polymethacrylate-based monolithic column provides a fast and efficient tool for microcystin separation.
• The developed capillary-HPLC method demonstrates high precision, efficiency, and sensitivity for routine monitoring of microcystins in environmental water samples.
• This approach shows significant potential for reliable and rapid detection of microcystins, contributing to water quality management.