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Published on: October 18, 2019
Analysis of hTERT expression in exfoliated cells from patients with bladder transitional cell carcinomas using SYBR
Xiao-Yan Xie1, Xin Yang, Jia-Hua Zhang
1Department of Urology, Southwest Hospital, Third Military Medical University, Chongqing 400038, PRC.
Aims:
To investigate whether hTERT mRNA expression could be used as a diagnostic and prognostic marker of bladder transitional cell carcinoma.
Methods:
SYBR green I real-time fluorescence quantitative PCR method was used to quantify hTERT mRNA expression in exfoliated cells from patients with bladder transitional cell carcinomas.
Results:
A significant correlation was observed between the level of hTERT mRNA expression in exfoliated cells collected in urine with pathological grade and clinical stage.
Conclusions:
Our results show that SYBR green real-time fluorescence quantitative evaluation of hTERT mRNA could be a useful marker of tumor progression for the early diagnosis and prognosis of bladder cancers.
