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Related Concept Videos

The Early Endosome: Endocytosis of Transferrin01:28

The Early Endosome: Endocytosis of Transferrin

Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...
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Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...

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Related Experiment Video

Updated: Jul 10, 2026

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo
12:42

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Published on: January 7, 2019

Development of a transferrin receptor-targeting HVJ-E vector.

Takashi Shimbo1, Masako Kawachi, Kotaro Saga

  • 1Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan.

Biochemical and Biophysical Research Communications
|October 27, 2007
PubMed
Summary

Researchers enhanced tumor-targeted drug delivery using modified hemagglutinating virus of Japan envelope (HVJ-E) vectors. Depleting HN protein and adding F-transferrin increased tumor targeting 32-fold, improving cancer therapy potential.

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Area of Science:

  • Biotechnology
  • Virology
  • Oncology

Background:

  • Tumor-targeted drug delivery is crucial for effective cancer therapy.
  • Hemagglutinating virus of Japan envelope (HVJ-E) vectors target cells via HN protein recognition of sialic acids.
  • Non-specific binding and targeting efficiency are limitations of current HVJ-E vectors.

Purpose of the Study:

  • To develop an improved HVJ-E vector with enhanced tumor-targeting efficiency.
  • To reduce non-specific binding of the HVJ-E vector.
  • To increase the efficacy of cancer drug delivery systems.

Main Methods:

  • Eliminated hemagglutinin-neuraminidase (HN) protein from HVJ-E using HN-specific short interfering RNA (siRNA).
  • Constructed a modified vector by depleting HN protein and incorporating a F-transferrin chimeric protein.
  • Incorporated Q-dots into modified vectors for quantitative analysis.

Main Results:

  • The modified HN-depleted HVJ vectors demonstrated significantly reduced non-specific binding.
  • The F-transferrin-containing modified vectors showed a 32-fold increase in tumor-targeting efficiency compared to wild-type HVJ-E.
  • Q-dot labeled modified vectors enabled precise measurement of targeting efficiency.

Conclusions:

  • HN protein depletion and F-transferrin incorporation effectively enhance HVJ-E vector tumor-targeting.
  • This modified vector represents a promising advancement for targeted cancer drug delivery.
  • Further research may lead to more effective and specific cancer treatment strategies.