Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Stringent Response in E. coli01:23

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
Inorganic Nitrogen Assimilation01:22

Inorganic Nitrogen Assimilation

Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme nitrate reductase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Horizontal Gene Transfer and Genome Rearrangements Shape Bacterial Adaptation for Bioremediation.

Environmental microbiology·2026
Same author

Author Correction: Engineering Salmonella as intracellular factory for effective killing of tumour cells.

Scientific reports·2026
Same author

Mechanochemical Fullerene Nanoencapsulation in Amino-Functionalized ZIF-12 for Visible-Light Disinfection of Waterborne Viruses and Bacteria.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Cu or Fe-Exchanged Natural Clinoptilolite as Sustainable Light-Assisted Catalyst for Water Disinfection at Near Neutral pH.

ChemPlusChem·2025
Same author

Mechanochemical synthesis of multivariate UPO-3 (Cu-ZIF-9-ica) MOF for inactivation of antibiotic-resistant bacteria and irrigation-quality water production via heterogeneous photo-Fenton catalysis.

Chemosphere·2025
Same author

Genetic and species rearrangements in microbial consortia impact biodegradation potential.

The ISME journal·2025

Related Experiment Video

Updated: Jul 10, 2026

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
11:35

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants

Published on: May 4, 2018

Transcriptome analysis of Pseudomonas putida in response to nitrogen availability.

Ana B Hervás1, Inés Canosa, Eduardo Santero

  • 1Centro Andaluz de Biología del Desarrollo/CSIC/Universidad Pablo de Olavide, Carretera de Utrera, Km. 1, 41013 Seville, Spain.

Journal of Bacteriology
|October 30, 2007
PubMed
Summary

Pseudomonas putida

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • Nitrogen metabolism regulation is crucial for bacterial survival.
  • Pseudomonas putida possesses complex regulatory networks for nutrient utilization.

Purpose of the Study:

  • To elucidate the regulatory network governing nitrogen assimilation in Pseudomonas putida.
  • To identify the key regulators controlling nutrient utilization pathways.

Main Methods:

  • Transcriptomic analysis to assess gene expression changes.
  • Biochemical assays to confirm enzyme activity.
  • Genetic manipulation to study regulatory mutants.

Main Results:

  • NtrC (Nitrogen regulatory protein C) identified as the master regulator of nitrogen metabolism.

More Related Videos

Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue
07:35

Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue

Published on: October 6, 2022

Related Experiment Videos

Last Updated: Jul 10, 2026

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
11:35

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants

Published on: May 4, 2018

Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue
07:35

Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue

Published on: October 6, 2022

  • NtrC activates genes for alternative nitrogen source assimilation.
  • NtrC represses carbon catabolism under nitrogen limitation.

    • Conclusions:

    • NtrC plays a dual role in nitrogen-limited Pseudomonas putida: promoting nitrogen uptake and inhibiting carbon breakdown.
    • This regulation prevents wasteful carbon and energy flux during nutrient scarcity.