Related Experiment Video
Updated: Jul 10, 2026
A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
[Determination of citrinin in red kojic by HPCE]
Jun Chen1, Zhang-wan Li, Wei Qi
1State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burns, Southwest Hospital, Third Military Medical University, Chongqing 400038, China. chenjpro@mail.tmmu.com.cn
Objective:
To establish an instant determination method of citrinin in red kojic by high performance capillary electrophorphores for the first time.
Method:
Red kojic was extracted with the mixtrue of Toluene and ethyl acetate (70:30). Separation was carried out in an uncoated fused silica capillary (50 microm x 45.0 cm). Meanwhile, a running voltage 15 kV, 20 mmol L(-1) borax buffer with 10.0 mmol L(-1) sodium deoxycholate (pH 9.3) and a UV detector at 212 nm were adopted.
Result:
Regression equation of citrinin was Y=9434 + 16781X (r =0.990), The lower limit of quantification (S/N > or =3) was 0.5 mg mL(-1). The assay coefficients of variation ranged from 98.8% to 101.1%. The intra and inter recovery ranged from 0.83 to 1.54% and from 1.86 to 5.09%. Twenty samples were determined with the method.
Conclusion:
The method is proved to be simple, rapid and accurate, and it can be used to determine citrinin in red kojic.
