[Histochemecal localization and the content compare of main medicinal components of vegetative organs in Bupleurum...

The paraffin sectioning, histochemistry and phytochemistry methods were applied in the study of the localization and the content changes of saponins and flavonoids in the vegetative organs in Bupleurum Chinense DC. The results showed that the saponins distributed in vascular cambium and secondary phloem of the root; In the stem, they distributed mainly in epiderm,collenchyma and the epithelial cells of the secretory canals which lied in cortex and pith; In the leaf, they distributed in the epiderm and spongy and palisade. However, flavonoids distributed in epiderm, collenchyma, cortex, pith path and myelin shealth cells of the stem; In the leaf, they were located mainly in the epiderm and the collenchyma. Meanwhile, the content of saponins in vegetative organs showed a changing law that the accumulation of them in the root occupied first place, the leaf came second and the stem was the lowest. But the content of flavonoids in leaf was higher than that in stem, and the content of them in stem was higher than that in root. Besides, the content of flavonoids in leaf was considerably high, thus it could offer basis for comprehensive utilization of Bupleurum Chinense DC. and made sense for both exploiting legitimately drug and conserving resource of Bupleurum Chinense DC.