Related Experiment Video
Updated: Jul 10, 2026

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
Published on: November 8, 2019
Enzymatic oxidation of aqueous pentachlorophenol
1Department of Chemical Engineering, The University of Seoul, Seoul 130-743, Korea. eykim@uos.ac.kr
Abstract:
The influence of the nonionic surfactant Tween 80 on pentachlorophenol (PCP) oxidation catalyzed by horseradish peroxidase was studied. The surfactant was tested at concentrations below and above its critical micelle concentration (CMC). Enhancement of PCP removal was observed at sub-CMCs. The presence of Tween 80 in the reaction mixture reduced enzyme inactivation which occurred through a combination of free radical attack and sorption by precipitated products. A simple first-order model was able to simulate time profiles for enzyme inactivation in the presence or absence of Tween 80. At supra-CMCs, the surfactant caused noticeable reductions in PCP removal, presumably through micelle partitioning of PCP which precluded the hydrophobic PCP molecule from interacting with the enzyme.
More Related Videos
Related Concept Videos
Oxidation of Phenols to Quinones
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox property is crucial in...
Hydrolysis of Chlorobenzene to Phenol: Dow Process
Oxidation of Alcohols
The process of oxidation in a chemical reaction is observed in any of the three forms:
Radical Autoxidation
Oxidation of Alkenes: Syn Dihydroxylation with Potassium Permanganate
Autoxidation of Ethers to Peroxides and Hydroperoxides

