Related Experiment Video
Updated: Jul 10, 2026

08:13
A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Immobilized cell augmented activated sludge process for enhanced nitrogen removal from wastewater
Rungrod Jittawattanarat1, Konstantinos Kostarelos, Eakalak Khan
1Department of Civil Engineering, Polytechnic University, Brooklyn, New York, USA.
Summary
The immobilized cell augmented activated sludge (ICAAS) system enhances nitrogen removal by 24% in wastewater treatment. This bioaugmentation method offers a viable upgrade for existing activated sludge systems.
Area of Science:
- Environmental Engineering
- Microbiology
Background:
- Activated sludge systems are crucial for wastewater treatment.
- Improving nitrogen removal efficiency is a key challenge in wastewater management.
Purpose of the Study:
- To investigate enhanced nitrogen removal using the immobilized cell augmented activated sludge (ICAAS) system.
- To evaluate the effectiveness of bioaugmentation with co-immobilized nitrifiers and denitrifiers.
Main Methods:
- Laboratory-scale batch enricher-reactors were used to cultivate nitrifying and denitrifying bacteria.
- Cellulose triacetate was employed as the immobilization medium for bacteria at a 2:1 mass ratio.
- The immobilized cells were used to augment a laboratory-scale completely mixed activated sludge system (CMAS) treating synthetic wastewater.
Main Results:
- The ICAAS system achieved 24% higher nitrogen removal compared to the control CMAS.
- The control CMAS demonstrated a nitrogen removal rate of 28% +/- 7%.
- Augmentation volumes of 5-20% by volume of co-immobilized cells significantly improved treatment performance.
Conclusions:
- The ICAAS system is a promising strategy for enhancing nitrogen removal in wastewater treatment.
- Bioaugmentation with immobilized nitrifiers and denitrifiers offers a viable method for upgrading existing activated sludge processes.
- The ICAAS approach provides a cost-effective solution for improving wastewater treatment efficiency.
Related Concept Videos
Biological Treatment of Effluent and Waste Water
Biological wastewater treatment relies on the metabolic activity of microorganisms to remove pollutants from sewage. In modern treatment systems, this process is organized into sequential stages that progressively reduce solid material, dissolved organic matter, and microbial contamination. Each stage plays a distinct role in improving water quality and preparing the effluent for safe discharge or reuse.Primary and Secondary TreatmentPrimary treatment is a physical process that removes large...
Microbial Wastewater Treatment
Microbial communities in aquatic ecosystems play a key role in the natural breakdown of contaminants introduced through domestic and industrial effluents. Acting as biological catalysts, these microbes change and mineralize a wide range of organic and inorganic pollutants under different redox conditions.In oxygen-rich surface waters, aerobic heterotrophs lead organic matter breakdown, using oxygen as the terminal electron acceptor to efficiently oxidize substrates to carbon dioxide and water.
Coagulation
Colloidal solids are solid particles suspended in solution. They are usually negatively charged, attracting a compact primary layer of positively charged ions, which attract more counterions to form an electrical double layer. Electrostatic repulsion between the charged double layers prevents the particles from colliding, stabilizing the colloids. These solids are often undesirable because they can contain toxins that are difficult to remove. Coagulation is a technique that helps aggregate and...
Biofuels
The microbial conversion of organic matter into biofuels holds potential as a renewable energy source. Among biofuel sources, microalgae are recognized as a highly efficient and adaptable feedstock for biodiesel production, owing to their rapid biomass accumulation, elevated lipid productivity, and capacity to proliferate in diverse aquatic systems, including freshwater, marine, and wastewater habitats. Unlike terrestrial crops, microalgae do not compete for land and can achieve significantly...
Bioremediation
Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.
Downstream Processing
Downstream processing begins once fermentation is complete and involves a series of steps to recover and purify products such as acids, vitamins, antibiotics, or proteins.Cell HarvestingFor example, for intracellular protein-based products, the first step is harvesting the cells. This is typically achieved using centrifugation or filtration to separate the cells from the liquid phase.Cell Disruption for Intracellular ProductsIf the target product is intracellular, the harvested cells must be...

